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Journal
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Immunology and Infection
/
无标记基因删除由絮凝血盒等位基因交换突变在
衣原体沙眼
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in
Chlamydia trachomatis
Please note that all translations are automatically generated.
Click here for the English version.
无标记基因删除由絮凝血盒等位基因交换突变在
衣原体沙眼
DOI:
10.3791/60848-v
•
10:35 min
•
January 30, 2020
•
Gabrielle Keb
,
Kenneth A. Fields
1
Department of Microbiology, Immunology, and Molecular Genetics
,
University of Kentucky
Chapters
00:04
Introduction
00:27
Design and Assembly of pSUmC-4.0 with Homology Arms Specific to the Gene of Interest
02:20
Transformation of
C. trachomatis
with pSUmC 4.0 + Homology Arms for Gene Deletion by Allelic Exchange
05:54
Clonal Isolation of Green-only
C. trachomatis
Deletion Mutant Containing the loxP Flanked Selection Cassette by Limiting Dilution
07:38
Transformation of
C. trachomatis
FRAEM Mutant with pSU-Cre to Initiate Removal of loxP Flanked Selection Cassette
08:42
Results: FLAEM Enables Markerless Gene Deletion in
C. trachomatis
and alleviates Cassette-induced Polar Effects
09:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文描述的是使用絮状盒状体等位基因突变(FLAEM)在
衣原体沙眼中
进行靶向、无标记基因删除的方法。
Tags
Markerless Gene Deletion
Floxed Cassette
Allelic Exchange Mutagenesis
Chlamydia Trachomatis
Homologous Recombination
PCR
DNA Assembly
E. Coli Transformation
McCoy Cells
Chlamydia Infection
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