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Biochemistry
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用细胞内快速光化学氧化蛋白质对细胞内蛋白质进行特征化
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins
Please note that all translations are automatically generated.
Click here for the English version.
用细胞内快速光化学氧化蛋白质对细胞内蛋白质进行特征化
DOI:
10.3791/60911-v
•
09:03 min
•
March 11, 2020
•
Emily E. Chea
,
Aimee Rinas
,
Jessica A. Espino
,
Lisa M. Jones
1
Department of Pharmaceutical Sciences
,
University of Maryland Baltimore
,
2
AIT Bioscience
Chapters
00:04
Introduction
00:39
In-Cell Fast Photochemical Oxidation of Proteins (IC-FPOP) System Assembly
02:53
Cell Preparation
03:44
IC-FPOP
05:40
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Analysis
06:21
Results: Representative IC-FPOP Characterization
08:14
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们使用称为细胞内快速光化学氧化蛋白质(IC-FPOP)的蛋白质足迹技术来描述活细胞中的蛋白质结构和相互作用位点。
Tags
In-cell FPOP
Protein-protein Interactions
Protein Structural Changes
Native Environments
Membrane Proteins
Diseases
Cancer
Genetic Disorders
Flow System
Syringe Pump
Laser Irradiation
Excimer Laser
Cell Culture
Cell Preparation
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