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Journal
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Chemistry
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将类似蛋白的蛋白质定向组装到定义的超分子结构和体外货物封装中
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Chemistry
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JoVE Journal
Chemistry
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
将类似蛋白的蛋白质定向组装到定义的超分子结构和体外货物封装中
DOI:
10.3791/60935-v
•
10:01 min
•
April 08, 2020
•
Andreas Schreiber*
1,2
,
Lara G. Stühn*
1,2
,
Süreyya E. Geissinger
2
,
Matthias C. Huber
2
,
Stefan M. Schiller
2,3,4,5,6
1
Center for Biological Systems Analysis
,
University of Freiburg
,
2
Faculty of Biology
,
University of Freiburg
,
3
Freiburg Institute for Advanced Studies (FRIAS)
,
University of Freiburg
,
4
BIOSS Centre for Biological Signalling Studies
,
University of Freiburg
,
5
IMTEK Department of Microsystems Engineering
,
University of Freiburg
,
6
Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies
,
University of Freiburg
Chapters
00:05
Introduction
00:45
Protein Expression and Preparation
03:38
Dye-modification of Elastin-like Proteins via SPAAC
04:44
THF Swelling Protocol
06:29
BuOH Extrusion Protocol
07:19
Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
09:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在有机溶剂和水性溶剂的界面上,定制的两栖乳素样蛋白质组装成复杂的超分子结构,如由环境参数触发的囊泡、纤维和焦质。所述装配协议产生具有可调特性的蛋白质膜型隔间 (PMBC),能够封装各种货物。
Tags
Elastin-like Proteins
Supramolecular Structures
Vesicles
Fibers
Coacervates
Protein Membrane-based Compartments
Phase Separation
Cargo Encapsulation
Unnatural Amino Acid
IPTG
Arabinose
Cell Lysis
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