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1Center for Biological Systems Analysis,University of Freiburg, 2Faculty of Biology,University of Freiburg, 3Freiburg Institute for Advanced Studies (FRIAS),University of Freiburg, 4BIOSS Centre for Biological Signalling Studies,University of Freiburg, 5IMTEK Department of Microsystems Engineering,University of Freiburg, 6Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies,University of Freiburg
Chapters
- 00:05Introduction
- 00:45Protein Expression and Preparation
- 03:38Dye-modification of Elastin-like Proteins via SPAAC
- 04:44THF Swelling Protocol
- 06:29BuOH Extrusion Protocol
- 07:19Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
- 09:22Conclusion
Op het snijvlak van organische en waterige oplosmiddelen assembleren op maat gemaakte amfifiele elastine-achtige eiwitten zich in complexe supramoleculaire structuren zoals blaasjes, vezels en coacervaten die worden geactiveerd door omgevingsparameters. De beschreven assemblageprotocollen leveren Op eiwitmembraan gebaseerde compartimenten (PMBC's) met tunable eigenschappen, waardoor de inkapseling van verschillende lading.
