Journal
/
Biology
/
从固定心脏分离心肌细胞进行免疫细胞化学和倍体分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis
Please note that all translations are automatically generated. Click here for the English version.

从固定心脏分离心肌细胞进行免疫细胞化学和倍体分析

DOI:
10.3791/60938-v

•

08:41 min

•

October 07, 2020

•

, ,
1Department of Integrative Biology and Physiology,University of Minnesota, 2Lillehei Heart Institute, Department of Medicine,University of Minnesota, 3Stem Cell Institute,University of Minnesota

Chapters

  • 00:04Introduction
  • 00:50Perfusion and Fixation of the Heart
  • 01:52Isolation of Fixed Cardiomyocytes
  • 03:17Image Quantification and Data Analysis
  • 04:53Data Analysis
  • 06:25Results: Segmentation and Classification of Isolated Cardiomyocytes
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

这项工作的目标是开发一种方法，从成人心脏中可重复分离心肌细胞，并测量DNA含量和核。

Tags

CardiomyocytesIsolationImmunocytochemistryPloidy AnalysisFixed HeartsPerfusionCollagenaseDissociationFiltrationCentrifugation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从胎盘中的造血干细胞的分离和分析

半自动小红桃光学心跳分析

从胚胎斑马鱼和cDNA合成的基因表达分析的RNA分离

在小鼠的心房和心室肌细胞的分离及钾离子通道的录音

分离新生小鼠心肌文化和

精子发生的细胞学分析：Live和固定制剂<em>果蝇</em>睾丸

鼠心肌细胞的分离及生理分析

隔离成年小鼠心肌细胞的细胞信号传导和文化及<em>在体外</em>心肌肥厚

隔离和线粒体从培养细胞和小鼠组织功能分析

分离培养和成年小鼠心肌细胞转导

Read Article