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免疫細胞化学とプロイド分析のための固定心からの心筋細胞の分離
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis
Please note that all translations are automatically generated.
Click here for the English version.
免疫細胞化学とプロイド分析のための固定心からの心筋細胞の分離
DOI:
10.3791/60938-v
•
08:41 min
•
October 07, 2020
•
Doğacan Yücel*
1,2
,
Jacob Solinsky*
2
,
Jop H. van Berlo
2,3
1
Department of Integrative Biology and Physiology
,
University of Minnesota
,
2
Lillehei Heart Institute, Department of Medicine
,
University of Minnesota
,
3
Stem Cell Institute
,
University of Minnesota
Chapters
00:04
Introduction
00:50
Perfusion and Fixation of the Heart
01:52
Isolation of Fixed Cardiomyocytes
03:17
Image Quantification and Data Analysis
04:53
Data Analysis
06:25
Results: Segmentation and Classification of Isolated Cardiomyocytes
08:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
本研究の目的は、心筋細胞を成体から再現的に単離し、DNAの含有量と核形成を測定する方法を開発することです。
Tags
Cardiomyocytes
Isolation
Immunocytochemistry
Ploidy Analysis
Fixed Hearts
Perfusion
Collagenase
Dissociation
Filtration
Centrifugation
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