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Isolering af kardiomyocytter fra faste hjerter til immunoktokemi og ploidy analyse
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

Isolering af kardiomyocytter fra faste hjerter til immunoktokemi og ploidy analyse

DOI:
10.3791/60938-v

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08:41 min

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October 07, 2020

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, ,
1Department of Integrative Biology and Physiology,University of Minnesota, 2Lillehei Heart Institute, Department of Medicine,University of Minnesota, 3Stem Cell Institute,University of Minnesota

Chapters

  • 00:04Introduction
  • 00:50Perfusion and Fixation of the Heart
  • 01:52Isolation of Fixed Cardiomyocytes
  • 03:17Image Quantification and Data Analysis
  • 04:53Data Analysis
  • 06:25Results: Segmentation and Classification of Isolated Cardiomyocytes
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med dette arbejde er at udvikle en metode til reproducerbart isolere kardiomyocytter fra det voksne hjerte og måle DNA-indhold og kerner.

Tags

CardiomyocytesIsolationImmunocytochemistryPloidy AnalysisFixed HeartsPerfusionCollagenaseDissociationFiltrationCentrifugation

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