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Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli
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Bioengineering
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JoVE Journal Bioengineering
Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

DOI:
10.3791/54397-v

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10:35 min

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August 13, 2016

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, , , , , ,
1Department of Agricultural Chemistry,National Taiwan University, 2Department of Physics,Fu-Jen Catholic University, 3Research Center for Applied Sciences,Academia Sinica

Chapters

  • 00:05Title
  • 01:03Chip Design and Fabrication
  • 01:57Chip Assembly
  • 04:37Cell Preparation and Experimental Setup
  • 06:02Experimental Setup
  • 07:32Data Analysis
  • 08:22Results: The Effects of Chemical Stress, Shear Stress, and Electrical Fields on Lung Cancer Cells
  • 09:47Conclusion

Summary

Automatic Translation

Automatic Translation

Micro-fabricated devices integrated with fluidic components provide an in vitro platform for cell studies mimicking the in vivo micro-environment. We developed polymethylmethacrylate-based microfluidic chips for studying cellular responses under single or coexisting chemical/electrical/shear stress stimuli.

Tags

Keywords: Microfluidic DevicesCellular ResponsesChemical StimuliElectrical StimuliShear StressPMMACarbon Dioxide Laser ScriberChemical-shear Stress ChipChemical-electric Field ChipPBSUV Sterilization

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