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通过预置等电聚焦(IEF)方法,将生物活性小分子、肽与天然来源和蛋白质从微生物中分离出来
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
DOI:

09:57 min

June 14, 2020

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Chapters

  • 00:04Introduction
  • 00:45Setting Up IEF Unit
  • 02:55IEF and Gymnema sylvestre
  • 04:17IEF and C. albicans
  • 06:45Gymnema sylvestre Bioactivity
  • 07:36Results: Isolating Proteins, Peptides, and Small Molecules
  • 09:21Conclusion

Summary

Automatic Translation

目标是通过使用液相同电聚焦(IEF)方法,分馏和分离生物活性小分子、复杂植物提取物中的肽和致病微生物中的蛋白质。此外,分离的分子被识别,其生物活性得到确认。

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