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Immunology and Infection

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JoVE Journal Immunology and Infection
Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
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Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Article DOI: 10.3791/61183-v • 08:44 min • May 30th, 2020
May 30th, 2020 •
1,2, 1,3, 1
1Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 3Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University

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Summary
Automatic Translation

Environmental allergens such as house dust mites (HDM) often contain microbial substances that activate innate immune responses to regulate allergic inflammation. The protocol presented here demonstrates the identification of dsRNA species in HDM allergens and characterization of their immunogenic activities in modulating eosinophilic lung inflammation.

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Identification Characterization Immunogenic RNA Species HDM Allergens Eosinophilic Lung Inflammation Microbial Substances Environmental Allergens Double-stranded RNA Species House Dust Mites In Vivo Modulate Techniques Simple Efficient Reproducible Commonly Used Tools And Equipment Lung Diseases Respiratory Viral Infections
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