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Parallell hög genomströmning Single Molecule Kinetic assay för Site-specifika DNA-klyvning
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Parallell hög genomströmning Single Molecule Kinetic assay för Site-specifika DNA-klyvning

Article DOI: 10.3791/61236-v • 06:51 min • May 6th, 2020
May 6th, 2020 •
1, 1, 2
1Department of Biology, Emmanuel College, 2Department of Chemistry and Physics, Emmanuel College

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En mycket parallell metod för att mäta den platsspecifika klyvningen av DNA på singelmolekylnivå beskrivs. Detta protokoll visar tekniken med hjälp av begränsningen endonuclease NdeI. Metoden kan enkelt modifieras för att studera vilken process som helst som resulterar i platsspecifik DNA-klyvning.

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Biologi DNA-tjudra enstaka molekyl DNA-klyvning protein-DNA-interaktioner begränsningsenonuclease platsspecifik DNA-bindning
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