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平行高通量单分子动力学测定,用于现场特异性DNA裂解
JoVE Journal
Biology
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JoVE Journal
Biology
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
Please note that all translations are automatically generated.
Click here for the English version.
平行高通量单分子动力学测定,用于现场特异性DNA裂解
DOI:
10.3791/61236-v
•
06:51 min
•
May 06, 2020
•
Emily K. Matozel
,
Nathaniel Dale
,
Allen C. Price
1
Department of Biology
,
Emmanuel College
,
2
Department of Chemistry and Physics
,
Emmanuel College
Chapters
00:04
Introduction
00:59
Making the Flow Cell
02:10
Tethering of DNA and Beads
05:16
Results: NdeI Cleavage is Dependent on Concentrations of Protein and Magnesium
06:14
Conclusion
Summary
Automatic Translation
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Automatic Translation
介绍了一种在单个分子水平上测量DNA的位点特异性裂解高度平行的方法。该协议演示了使用限制内核糖 NdeI 的技术。该方法可以很容易地修改,以研究任何过程,导致现场特异性DNA裂解。
Tags
DNA Cleavage
Single Molecule Kinetics
High Throughput
DNA Binding
DNA Modification
DNA Target Search
Flow Cell
Tethering
PCR
Buffer A
Anti-digoxigenin
Fab Fragments
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