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Journal
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Biochemistry
/
测量小核苷酸基质的多核苷酸磷酸化的非放射性测定
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Biochemistry
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JoVE Journal
Biochemistry
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
Please note that all translations are automatically generated.
Click here for the English version.
测量小核苷酸基质的多核苷酸磷酸化的非放射性测定
DOI:
10.3791/61258-v
•
06:49 min
•
May 08, 2020
•
Monica C. Pillon
,
Robin E. Stanley
1
Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services
,
National Institutes of Health
Chapters
00:05
Introduction
00:29
In Vitro RNA Kinase Reaction
01:27
Gel Electrophoresis
04:53
Results: Quantification of RNA Phosphorylation
06:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种非放射性检测，用于测量小DNA和RNA基质上多核苷酸激酶（PNKs）的激酶活性。
Tags
Keywords: Polynucleotide Kinase
Phosphorylation
DNA
RNA
Substrate
Nonradioactive Assay
Acrylamide Gel
Denaturing Gel
Urea
TBE Buffer
RNA Enzyme Kinase Reactions
ATP Substrate
Quenching
Downstream Analysis
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