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JoVE Journal Biochemistry
Essai non radioactif pour mesurer la phosphorylation polynucléotide des petits substrats nucléotides
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Essai non radioactif pour mesurer la phosphorylation polynucléotide des petits substrats nucléotides

Article DOI: 10.3791/61258-v • 06:49 min • May 8th, 2020
May 8th, 2020 •
1, 1
1Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services, National Institutes of Health

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Ce protocole décrit un test non radioactif pour mesurer l’activité de kinase des kinases de polynucléotide (PNK) sur de petits substrats d’ADN et d’ARN.

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Biochimie numéro 159 polynucléotide kinase gel polyacrylamide réaction de transfert de phosphore phosphorylation d’ARN Grc3 essai non radiotactive
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