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Journal / Neuroscience / Dissektion og isolering af Murine Glia fra flere…
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Neuroscience

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JoVE Journal Neuroscience
Dissektion og isolering af Murine Glia fra flere centralnervesystemregioner
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Dissektion og isolering af Murine Glia fra flere centralnervesystemregioner

Article DOI: 10.3791/61345-v • 08:00 min • June 4th, 2020
June 4th, 2020 •
1, 1,2
1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute, Kent State University

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Her præsenterer vi en protokol for in vitro-isolering af flere gliacellepopulationer fra en mus CNS. Denne metode giver mulighed for adskillelse af regionale microglia, oligodendrocytprecursorceller og astrocytter for at studere fænotyperne af hver i en række kultursystemer.

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Neurovidenskab udgave 160 glia regional heterogenitet oligodendrocytforløbercelle microglia astrocyt in vitro-kultur immunocytokemi
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