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Journal / Neuroscience / Subcellulær fraktionering til isolering af…
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Neuroscience

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JoVE Journal Neuroscience
Subcellulær fraktionering til isolering af synaptiske komponenter fra Murine-hjernen
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Subcellulær fraktionering til isolering af synaptiske komponenter fra Murine-hjernen

Article DOI: 10.3791/64574-v • 12:14 min • September 14th, 2022
September 14th, 2022 •
1,2,3, 1,2, 1,2
1Department of Neurology, Yale University, 2Department of Neuroscience, Yale University, 3Interdepartmental Neuroscience Program, Yale University

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Denne protokol præsenterer en robust, detaljeret metode til at opnå meget rene synaptosomer, synaptiske vesikler og andre synaptiske fraktioner fra musehjernen. Denne metode muliggør evaluering af synaptiske processer, herunder biokemisk analyse af proteinlokalisering og funktion med rumopløsning.

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Neurovidenskab udgave 187
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