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En tyngdekraftsfodret transkardieperfusionsmetode til histologisk analyse af musens centralnervesystem
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

En tyngdekraftsfodret transkardieperfusionsmetode til histologisk analyse af musens centralnervesystem

DOI:
10.3791/63386-v

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10:37 min

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January 21, 2022

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, ,
1Department of Biochemistry and Molecular Biology,State University of New York Upstate Medical University, 2Department of Neurology,State University of New York Upstate Medical University, 3Department of Neuroscience and Physiology,State University of New York Upstate Medical University

Chapters

  • 00:04Introduction
  • 00:46Construction of Perfusion Apparatus and Dissection Platform
  • 02:40Preparation of the Paraformaldehyde Solution
  • 04:58Transcardial “Pump-Free” Perfusion
  • 09:18Results: Histological Analysis Following Gravity-Fed Perfusion
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

En bekvem tyngdekraftsfodret perfusionsmetode til histologisk analyse af musens centralnervesystem præsenteres. Immunofluorescerende påvisning af phosphoryleret α-synuclein er demonstreret i en musemodel af Parkinsons sygdom. Dette arbejde beskriver også omfattende transkardieperfusion, dissektion, vævsfrysning / indlejring og frosne sektioneringstrin.

Tags

Gravity-fed Transcardial PerfusionHistologic AnalysisMouse Central Nervous SystemFixative PerfusionProtocolApparatusBuffer BottlesMicro SpatulasTubingSyringePBSPFAStyrofoam Block

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