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Biochemistry
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细胞性谷胱甘肽S-转移酶潜在抑制剂的分光光度筛选
JoVE Journal
Biochemistry
Author Produced
This content is Free Access.
JoVE Journal
Biochemistry
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
Please note that all translations are automatically generated.
Click here for the English version.
细胞性谷胱甘肽S-转移酶潜在抑制剂的分光光度筛选
DOI:
10.3791/61347-v
•
14:57 min
•
October 10, 2020
•
Shannon K. D. Robin
2
,
Marc Ansari*
1,3
,
Chakradhara Rao S. Uppugunduri*
1,3
1
Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine
,
University of Geneva
,
2
Section of Biology, Faculty of Science
,
University of Geneva
,
3
Onco-Hematology Unit, Department of Women, Children-Adolescents
,
University Hospitals of Geneva
Chapters
00:00
Introduction
00:56
Preparation of the Enzymatic Solution
03:10
Michaelis-Menten Constant of CDNB with GST
04:42
Absorbance of Potential GST Inhibitors
06:08
Determination of the IC50
07:49
Determination of the Type of Inhibition and Ki
09:46
Results: Testing the Potential of Curcumin as a GST Inhibitor
13:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
谷胱甘肽S-转移酶(GSTs)是参与许多化疗药物代谢的解毒酶。GSTs的表达与癌症化疗的抗性相关。对抗这种表型的一个方法就是使用抑制剂。该协议描述了一种使用分光光度测定来筛选潜在GST抑制剂的方法。
Tags
Spectrophotometric Screening
Cytosolic Glutathione S-transferases
GST Inhibitors
Enzymatic Activity
Inhibitory Concentration
Mode Of Inhibition
CDNB
GSH
PBS
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