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Spektrofotometrisk screening for potentielle hæmmere af cytosol glutathion S-transferaser
JoVE Journal
Biochemistry
Author Produced
This content is Free Access.
JoVE Journal Biochemistry
Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
DOI:

14:57 min

October 10, 2020

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Chapters

  • 00:00Introduction
  • 00:56Preparation of the Enzymatic Solution
  • 03:10Michaelis-Menten Constant of CDNB with GST
  • 04:42Absorbance of Potential GST Inhibitors
  • 06:08Determination of the IC50
  • 07:49Determination of the Type of Inhibition and Ki
  • 09:46Results: Testing the Potential of Curcumin as a GST Inhibitor
  • 13:56Conclusion

Summary

Automatic Translation

Glutathion S-transferaser (GSTs) er afgiftning enzymer involveret i metabolismen af talrige kemoterapeutiske lægemidler. Overekspression af GST'er er korreleret med kræft kemoterapi resistens. En måde at imødegå denne fænotype er at bruge hæmmere. Denne protokol beskriver en metode, der anvender en spektrofotometrisk analyse til skærmen for potentielle GST-hæmmere.

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