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Spektrofotometrisk screening for potentielle hæmmere af cytosol glutathion S-transferaser
This content is Free Access.
1Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine,University of Geneva, 2Section of Biology, Faculty of Science,University of Geneva, 3Onco-Hematology Unit, Department of Women, Children-Adolescents,University Hospitals of Geneva
Chapters
- 00:00Introduction
- 00:56Preparation of the Enzymatic Solution
- 03:10Michaelis-Menten Constant of CDNB with GST
- 04:42Absorbance of Potential GST Inhibitors
- 06:08Determination of the IC50
- 07:49Determination of the Type of Inhibition and Ki
- 09:46Results: Testing the Potential of Curcumin as a GST Inhibitor
- 13:56Conclusion
Glutathion S-transferaser (GSTs) er afgiftning enzymer involveret i metabolismen af talrige kemoterapeutiske lægemidler. Overekspression af GST'er er korreleret med kræft kemoterapi resistens. En måde at imødegå denne fænotype er at bruge hæmmere. Denne protokol beskriver en metode, der anvender en spektrofotometrisk analyse til skærmen for potentielle GST-hæmmere.
