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有機溶媒混合物中の複雑なナノ構造へのガンマ修飾ペプチド核酸の自己集合
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
Please note that all translations are automatically generated.
Click here for the English version.
有機溶媒混合物中の複雑なナノ構造へのガンマ修飾ペプチド核酸の自己集合
DOI:
10.3791/61351-v
•
08:15 min
•
June 26, 2020
•
Sriram Kumar
,
Ying Liu
,
Rebecca E. Taylor
2,3
1
Department of Mechanical Engineering
,
Carnegie Mellon University
,
2
Department of Biomedical Engineering
,
Carnegie Mellon University
,
3
Department of Electrical and Computer Engineering
,
Carnegie Mellon University
Chapters
00:04
Introduction
00:42
Self-assembly Protocol for Multiple Distinct PNA Oligomers
02:48
Total Internal Reflection Fluorescence (TIRF) Microscopy Imaging
05:08
Different Morphologies for PNA Nanofibers in Varying Concentrations of SDS
06:01
Results: TIRF and TEM Imaging of PNA Nanofibers
07:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本稿では、有機溶媒混合物中のガンマ修飾ペプチド核酸オリゴマーからのナノ構造の設計と自己集合のためのプロトコルを提供する。
Tags
Gamma-modified Peptide Nucleic Acids
Nanostructures
Self-assembly
Organic Solvent Mixtures
DMSO
DMF
1
4-dioxane
Thermal Annealing
Nitrocellulose
Humidity Chamber
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