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JoVE Journal
Immunology and Infection

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Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Article DOI: 10.3791/61376-v 08:33 min August 5th, 2020
August 5th, 2020



This live-bacterial cell imaging protocol allows for visualization of interactions between multiple bacterial species at the single-cell level over time. Time-lapse imaging allows for observation of each bacterial species in monoculture or coculture to interrogate interspecies interactions in multispecies bacterial communities, including individual cell motility and viability.


Kinetic Visualization Single-cell Interspecies Bacterial Interactions Tracking Gene Expression Viability Staining Agarose Pads Low Melt Agarose Bacterial Growth Media Silicon Cut Out 35 Millimeter Dish Microscope Environmental Chamber Humidity Wipes Lint-free Wipe Sterile Petri Dish
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