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Kinetisk visualisering af encellede interarts bakterielle interaktioner
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
DOI:

08:33 min

August 05, 2020

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Chapters

  • 00:04Introduction
  • 00:34Preparation of Agarose Pads
  • 01:28Equilibrating Materials to Experimental Temperature
  • 02:06Inoculation of Agarose Pads
  • 03:13Microscope Setup for Live Imaging
  • 05:32Results: P. aeruginosa Behavior when Cocultured with S. aureus
  • 07:47Conclusion

Summary

Automatic Translation

Denne levende bakterielle cellebilleddannelse protokol giver mulighed for visualisering af interaktioner mellem flere bakteriearter på encellet niveau over tid. Time-lapse imaging giver mulighed for observation af hver bakterieart i monokultur eller coculture at afhøre interspecies interaktioner i multispecies bakterielle samfund, herunder individuelle celle motilitet og levedygtighed.

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