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Kinetisk visualisering af encellede interarts bakterielle interaktioner
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Chapters
- 00:04Introduction
- 00:34Preparation of Agarose Pads
- 01:28Equilibrating Materials to Experimental Temperature
- 02:06Inoculation of Agarose Pads
- 03:13Microscope Setup for Live Imaging
- 05:32Results: P. aeruginosa Behavior when Cocultured with S. aureus
- 07:47Conclusion
Denne levende bakterielle cellebilleddannelse protokol giver mulighed for visualisering af interaktioner mellem flere bakteriearter på encellet niveau over tid. Time-lapse imaging giver mulighed for observation af hver bakterieart i monokultur eller coculture at afhøre interspecies interaktioner i multispecies bakterielle samfund, herunder individuelle celle motilitet og levedygtighed.
