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Journal
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Immunology and Infection
/
非肝细胞293T-NE-3NRS细胞中乙型肝炎病毒感染的建模
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
Please note that all translations are automatically generated.
Click here for the English version.
非肝细胞293T-NE-3NRS细胞中乙型肝炎病毒感染的建模
DOI:
10.3791/61414-v
•
09:02 min
•
June 05, 2020
•
Xiaoyue Sun*
1,2,3
,
Xiaoqiang Yang*
1,2,3,4
,
Chui Zeng
2,3
,
Fayed Attia Koutb Megahed
2,3,5
,
Qi Zhou
2,3
,
Tingdang Liu
2,3
,
Waqas Iqbal
2,3
,
Pingnan Sun
2,3
,
Xiaoling Zhou
2,3
1
Stem Cell Research Center
,
Shantou University Medical College
,
2
The Center for Reproductive Medicine
,
Shantou University Medical College
,
3
Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology
,
Shantou University Medical College
,
4
Medical Research Center, Sun Yat-Sen Memorial Hospital
,
Sun Yat-Sen University
,
5
Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute
,
General Autority – City of Scientific Researches and Technological Applications
Chapters
00:04
Introduction
01:36
HepG2.2.15 Cell Culture
03:04
Detection of HBV DNA in Supernatant Concentrate
04:23
In Vitro Infection of HepG2-NE and 293T-NE-3NRs Cells
05:27
HepG2.2.15 Co-culture with HepG2-NE / 293T-NE-3NRs / 293T-NE
06:41
Results: HBcAg Expression in 293T-NE, 293T-NE-3NRs and HepG2-NE
07:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本手稿描述了新型工程293T细胞(293T-NE-3NRS,表达人类NTCP、HNF4+、RXR+和PAR+)和传统肝细胞(HepG2-NE,表达人类NTCP)中乙型肝炎病毒(HBV)感染的详细方案。
Tags
Hepatitis B Virus
HBV
Non-hepatic Cells
293T-NE-3NR
In Vitro Model
HepG2.2.15
Cell Culture
Viral Replication
Extrahepatic Syndromes
Cell Line Engineering
Biosafety
Viral Supernatant
Virus Concentration
Real-time PCR
Article
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