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Immunology and Infection

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Modellering hepatitis B-virusinfectie in niet-leverloze 293T-NE-3NRs-cellen

Article DOI: 10.3791/61414-v • 09:02 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3,4, 1,2,3, 1,2,3,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications
* These authors contributed equally

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Dit manuscript beschrijft een gedetailleerd protocol voor hepatitis B-virus (HBV) infectie in nieuwe 293T cellen (293T-NE-3NRs, uitdrukken van menselijke NTCP, HNF4α, RXRα en PPARα) en traditionele levercellen (HepG2-NE, uitdrukken van menselijke NTCP).

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Deze maand in JoVE HBV infectie model HepG2.2.15 HepG-NE 293T-NE-3NRs NTCP nucleaire hormoonreceptoren
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