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Journal / Immunology and Infection / Vurdering af udtryk for major histokompatibilitet …
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Vurdering af udtryk for major histokompatibilitet Complex Klasse I på primær Murine Hippocampal Neurons af Flow Cytometry
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Vurdering af udtryk for major histokompatibilitet Complex Klasse I på primær Murine Hippocampal Neurons af Flow Cytometry

Article DOI: 10.3791/61436-v • 08:07 min • May 19th, 2020
May 19th, 2020 •
1, 1
1Department of Biological Sciences, University of North Carolina at Charlotte

Summary
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Denne protokol beskriver en metode til dyrkning af primære hippocampale neuroner fra embryonale musehjerne. Udtrykket af større histokompatibilitet kompleks klasse I på den ekstracellulære overflade af kulturperlede neuroner vurderes derefter ved flow cytometrisk analyse.

Tags

Immunologi og infektion Problem 159 antigenpræsentation flowcytometry hippocampus større histokompatibilitetskompleks klasse I mikrodissection neuroimmun primære neuroner
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