JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
按流细胞测量评估主要穆林河马神经元的主要组织相容性综合类 I 的表达
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
按流细胞测量评估主要穆林河马神经元的主要组织相容性综合类 I 的表达
DOI:
10.3791/61436-v
•
08:07 min
•
May 19, 2020
•
Kristen E. Funk
,
Sarah K. Lotz
1
Department of Biological Sciences
,
University of North Carolina at Charlotte
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了一种从胚胎小鼠大脑中培养原发性海马神经元的方法。然后通过流动细胞学分析对培养神经元细胞外表面的主要组织相容性复杂I类的表达进行评估。
Tags
Flow Cytometry
MHC Class I
Primary Neurons
Hippocampus
Embryonic Brain Dissection
Papain Dissociation
Hibernate-E Medium
Trituration
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
流式细胞仪分析小鼠主动脉内流的免疫细胞
人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具
检测荧光纳米粒子的相互作用与原发性免疫细胞亚群流式细胞仪的
原位
检测自身反应性CD4 + T细胞在大脑和心脏使用主要组织相容性复合II类Dextramers的
通过流式细胞仪大鼠肾脏巨噬细胞的表型特征
荧光激活细胞分选从小鼠骨髓浆树突状细胞的分离纯化
评估视网膜小胶质细胞吞噬功能<em>在体内</em>使用基于流式细胞术流量测定
荧光为基础的神经氨酸酶抑制测定流感病毒的易感性评估抗病毒药的神经氨酸酶抑制剂类
小鼠与人肺泡巨噬细胞的分离和
体外
培养
全血流式细胞术分析人单核细胞亚群的表征
Read Article