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Biomimetic Replication of Root Surface Microstructure using Alteration of Soft Lithography
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Biomimetic Replication of Root Surface Microstructure using Alteration of Soft Lithography

Biomimetic Replication of Root Surface Microstructure using Alteration of Soft Lithography

DOI:
10.3791/61437-v

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05:53 min

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August 05, 2020

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, ,
1Institute of Plant Sciences,Agricultural Research Organization (Volcani Center), 2Agro-NanoTechnology and Advanced Materials Center,Agricultural Research Organization (Volcani Center)

Chapters

  • 00:04Introduction
  • 00:51Root Preparation
  • 01:37Preparation of the Root Negative Replica from Polyurethane
  • 02:47Preparation of the Root Positive Replica from PDMS and Ethyl Cellulose
  • 04:05Results: Microscopy Images of Negative and Positive Replica
  • 05:14Conclusion

Summary

Automatic Translation

Automatic Translation

Biomimetics has been previously used as a tool to study leaf-microorganism interactions. However, no such tool exists for roots. Here, we develop a protocol to form synthetic surfaces mimicking root surface microstructure for the study of root-environment interactions.

Tags

Keywords: Biomimetic ReplicationRoot Surface MicrostructureSoft LithographyRoot-environment InteractionsNegative MoldAdventitious RootsSeed Germinated RootsDiurethane DimethacrylateEthyl MethacrylatePlasticizerPhoto InitiatorPDMS (polydimethylsiloxane)Positive Replica

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