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Kultur og assay af storstilet blandet stadie Caenorhabditis elegans Populationer
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations
DOI:

08:51 min

May 05, 2021

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Chapters

  • 00:04Introduction
  • 00:28Large-Scale Culture Plate (LSCP) Gravid Adult Spot Bleaching
  • 01:21LSCP Sample Harvest
  • 03:02Population Size Estimation
  • 04:19Large Particle Flow Cytometry (LPFC) Preparation
  • 05:27Population Distribution Documentation and 384-Well Imaging Plate Preparation
  • 06:24Results: Representative Large-Scale Mixed-Stage C. elegans Population Evaluation
  • 08:06Conclusion

Summary

Automatic Translation

For at bruge Caenorhabditis elegans (C. elegans) i omics forskning, en metode er nødvendig for at generere store populationer af orme, hvor en enkelt prøve kan måles på tværs af platforme for sammenlignende analyser. Her præsenteres en metode til kultur C. elegans populationer på store kulturplader (LSCP'er) og til at dokumentere befolkningstilvæksten.

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