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通过特洛多毒素微注射可逆灭活,揭开鼠脑离散区在排卵调节中的作用
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
DOI:

11:04 min

September 03, 2020

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Chapters

  • 00:04Introduction
  • 00:47Construction of Bilateral Cannulas, Obturators, and Caps
  • 02:49Construction of Microinjectors
  • 04:10Animal Maintenance and Vaginal Smears
  • 05:31Microinjection of TTX or Vehicle Solutions into Freely Moving Rats
  • 07:27Tissue Processing
  • 08:44Results: Percentage of Ovulating Animals and Median of the Number of Ova Shed after Microinjection with Artificial Cerebrospinal Fluid (ACSF) or Tetrodotoxin (TTX)
  • 10:08Conclusion

Summary

Automatic Translation

本协议描述了低成本微注射系统的构建、对深脑结构的立体定向植入以及清醒和无拘无束的大鼠对四毒素定时微注射的程序。目标是通过抑制下丘脑结构的神经活动来揭示下丘脑结构参与排卵的调节。

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