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Journal / Developmental Biology / 静脈奇形に対する患者由来異種移植片モデル…
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Developmental Biology

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JoVE Journal Developmental Biology
静脈奇形に対する患者由来異種移植片モデル
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
Journal (Developmental Biology)
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
Journal (Developmental Biology)
3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
 
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静脈奇形に対する患者由来異種移植片モデル

Article DOI: 10.3791/61501-v • 06:51 min • June 15th, 2020
June 15th, 2020 •
*1, *1, 1,2
1Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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静脈奇形のマウス異種移植片モデルを生成するための詳細なプロトコルを提示する。このモデルは、超活性化TIE2および/またはPIK3CA遺伝子変異を含む患者由来内皮細胞の皮下注射に基づいている。異種移植病変はVM患者組織の組織病理学的特徴を密接に再現する。

Tags

発生生物学,課題 160 静脈奇形 異種移植片 内皮細胞 TIE2 PIK3CA 血管病変
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