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JoVE Journal Biology

Situハイブリダイゼーションにおける2色単分子蛍光を用いた 大腸菌 における空間と時間におけるmRNA運動学の探査

A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ

Journal (Biology)

A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

Journal (Biology)

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

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Situハイブリダイゼーションにおける2色単分子蛍光を用いた大腸菌における空間と時間におけるmRNA運動学の探査

Article DOI: 10.3791/61520-v • 10:01 min • July 30th, 2020

July 30th, 2020 •

Sangjin Kim^1,2,3,4, Kavya Vaidya^1,2

¹Department of Physics, University of Illinois at Urbana-Champaign, ²Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, ³Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, ⁴Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign

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このプロトコルは、mRNA合成および分解のインビボ運動を測定するための一分子蛍光をその場所でのハイブリダイゼーション(smFISH)での適用について説明する。

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生物学,課題 161 smFISH RNA イメージング mRNA の局在化単一細胞単一分子転写転写伸び早期終了 mRNA 分解

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