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Journal / Biology / のタンパク質複合体の同定
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のタンパク質複合体の同定
Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
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Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
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のタンパク質複合体の同定

Article DOI: 10.3791/4057-v • 14:58 min • November 12th, 2012
November 12th, 2012 •
1,2, 1, 1, 1,3, 1,3
1Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, 2Deparment of Biochemistry, Research and Innovation Centre, University of Regina, 3Department of Medical Genetics and Microbiology, University of Toronto

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マススペクトロメトリー（APMS）との組み合わせでタグ付けされたタンパク質のアフィニティー精製は、タンパク質相互作用ネットワークの体系的マッピングのため、生物学的プロセスの基本メカニズムを調査するための強力な方法です。ここでは、細菌のために開発最適化された順次ペプチドアフィニティー（SPA）をAPMSの手順を説明

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遺伝学、問題69、分子生物学、医学、生化学、微生物学、アフィニティー精製、
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