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Modelo de lesão renal aguda induzida por cisplatina em zebrafish adulto
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Modelo de lesão renal aguda induzida por cisplatina em zebrafish adulto

Article DOI: 10.3791/61575-v • 13:25 min • May 15th, 2021
May 15th, 2021 •
*1, *1, *1,2, 1, 1, 1, 1,2
1Department of Immunology, University of São Paulo, 2Department of Medicine, Nephrology Division, Federal University of São Paulo
* These authors contributed equally

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Este protocolo descreve os procedimentos para induzir lesão renal aguda (AKI) em zebrafish adulto usando cisplatina como um agente nefrotóxico. Detalhamos as etapas para avaliar a reprodutibilidade da técnica e duas técnicas para analisar inflamação e morte celular no tecido renal, citometria de fluxo e TUNEL, respectivamente.

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Medicina Questão 171 Rim Lesão Renal Aguda (AKI) Cisplatina Zebrafish Inflamação Citometria de Fluxo TUNEL Morte Celular
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