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Chapters
- 00:05Title
- 01:46Preparation of Morpholino and Injection Apparatus
- 03:44Preparation of Fertilized Zebrafish Embryos and Injection with Morpholinos
- 04:55Phenotype Rescue Experiments
- 06:42Florescent Imaging of Injected Embryos
- 07:55Results: Analysis of wnt5a Morpholino Knockdown in Zebrafish
- 10:09Conclusion
We describe a method of generating a possible zebrafish model of polycystic kidney disease. We used Tg(wt1b:GFP) fish to visualize kidney structure. Knockdown of wnt5a was by morpholino injection. Pronephric cyst formation after wnt5a knockdown was observed in this GFP transgenic zebrafish.
