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Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Article DOI: 10.3791/61578-v 10:59 min January 28th, 2021
January 28th, 2021



SEC-BioSAXS measurements of biological macromolecules are a standard approach for determining solution structure of macromolecules and their complexes. Here, we analyze SEC-BioSAXS data from two types of commonly encountered SEC traces—chromatograms with fully resolved and partially resolved peaks. We demonstrate the analysis and deconvolution using scatter and BioXTAS RAW.


Keywords: SEC-SAXS EFA Deconvolution Scatter Vaccinia Virus DNA Polymerase Background Subtraction Size Exclusion Chromatography Monodispersity Macromolecule Macromolecular Complex Deconvolution Data Analysis
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