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Journal / Immunology and Infection / Visualisierung von Pseudomonas aeruginosa im…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisierung von Pseudomonas aeruginosa im Sputum von Mukoviszidose-Patienten
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Visualisierung von Pseudomonas aeruginosa im Sputum von Mukoviszidose-Patienten

Article DOI: 10.3791/61631-v • 07:25 min • July 16th, 2020
July 16th, 2020 •
1, 2, 1, 1, 1,3, 1,4
1Translational Medicine, Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

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Dieses Protokoll bietet Methoden zur Visualisierung von Bakterienzellen und Polysaccharid-Syntheselocus (Psl)-Polysacchariden im Sputum von Mukoviszidose-Patienten.

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Immunologie und Infektion Ausgabe 161 Chronische Infektion Lungenfunktion Morbidität Antibiotikatherapie Pädiatrische Patienten Räumliche Organisation Aggregation Biofilmbildung Polysaccharidproduktion Sputumproben Gewebereinigungstechnik Hydrogelmatrix 3D-Strukturen Fluoreszenzmarkierungen Fluoreszenz-in-situ-Hybridisierung Anti-PSL-Antikörper Exopolysaccharide DAPI-Färbung Wirtszellen hochauflösende Bildgebung
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