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JoVE Journal
Developmental Biology

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使用紫罗兰兴奋细胞渗透DNA结合染料分离穆林精子生成细胞
 
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使用紫罗兰兴奋细胞渗透DNA结合染料分离穆林精子生成细胞

Article DOI: 10.3791/61666-v 08:21 min January 14th, 2021
January 14th, 2021

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Summary

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Click here for the English version.

在这里,我们提出了一个简单而有效的方法来从成年小鼠睾丸中分离活的美病细胞和后美生菌细胞。使用低细胞毒性、紫罗兰兴奋的DNA结合染料和荧光激活细胞分选,可以分离高度丰富的精子致原细胞群,用于许多下游应用。

Tags

发育生物学, 问题 167, 精子生成, 美病, 精子细胞, 精子, 荧光激活细胞排序 (FACS), 染料环紫罗兰 (DCV)
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