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Developmental Biology
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从小鼠面部过程和培养的腭间充质细胞中分离全细胞蛋白质裂解物以进行磷酸化蛋白分析
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JoVE Journal
Developmental Biology
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠面部过程和培养的腭间充质细胞中分离全细胞蛋白质裂解物以进行磷酸化蛋白分析
DOI:
10.3791/63834-v
•
07:26 min
•
April 01, 2022
•
Madison A. Rogers*
1
,
Brenna J. C. Dennison*
1
,
Katherine A. Fantauzzo
1
Department of Craniofacial Biology, School of Dental Medicine
,
University of Colorado Anschutz Medical Campus
Chapters
00:04
Introduction
00:48
Harvesting E11.5 Mouse Embryos
02:31
Dissecting Maxillary Processes from E11.5 Mouse Embryos
04:12
Isolating Whole Cell Protein Lysates from Mouse Maxillary Processes
05:06
Isolating Whole Cell Protein Lysates from Primary and/or Immortalized Mouse Embryonic Palatal Mesenchyme Cells
06:22
Results: Assessing Phosphorylated Protein Levels in Cell Lysates from Cultured Mouse Embryonic Palatal Mesenchyme Cells
06:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提出了一种从解剖的小鼠胚胎面部过程或培养的小鼠胚胎腭间充质细胞中分离全细胞蛋白裂解物并随后进行蛋白质免疫印迹以评估磷酸化蛋白水平的方法。
Tags
Protein Lysates
Phosphoprotein Analysis
Facial Processes
Palatal Mesenchyme Cells
Craniofacial Development
Mouse Embryos
Protein Isolation
Phosphatase Inhibitors
Protein Phosphorylation
Dissection
Cell Culture
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