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Visualisering af gentagne møder med en antigenmærket blokerende læsion
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Visualisering af gentagne møder med en antigenmærket blokerende læsion

Article DOI: 10.3791/61689-v • 08:24 min • July 27th, 2021
July 27th, 2021 •
*1, *2, 1, 3, 1, 4, 5, 6, 1, 1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 2Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, 3Department of Molecular Biology and Genetics, Cornell University, 4Department of Cellular and Molecular Medicine, University of Copenhagen, 5Horizon Discovery, 6Boston University School of Medicine
* These authors contributed equally

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Mens replikationsgaffelkollisioner med DNA-addukter kan fremkalde dobbeltstrengsbrud, er der mindre kendt om interaktionen mellem replisomer og blokerende læsioner. Vi har anvendt nærhedsligationsanalysen til at visualisere disse møder og til at karakterisere konsekvenserne for replikerende sammensætning.

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Biologi udgave 173 replisome blokerende læsion PLA nærhedsligationsanalyse DNA-interstreng tværbinding antigen-tagged tværbindinger
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