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Visualisering av replisome møter med et antigen merket blokkerende lesjon
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Visualisering av replisome møter med et antigen merket blokkerende lesjon

Article DOI: 10.3791/61689-v • 08:24 min • July 27th, 2021
July 27th, 2021 •
*1, *2, 1, 3, 1, 4, 5, 6, 1, 1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 2Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, 3Department of Molecular Biology and Genetics, Cornell University, 4Department of Cellular and Molecular Medicine, University of Copenhagen, 5Horizon Discovery, 6Boston University School of Medicine
* These authors contributed equally

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Mens replikasjonsgaffelkollisjoner med DNA-addukter kan indusere dobbeltstrengbrudd, er mindre kjent om samspillet mellom replisomes og blokkerende lesjoner. Vi har benyttet nærhetsligeringsanalysen for å visualisere disse møtene og for å karakterisere konsekvensene for replisome sammensetning.

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Biologi utgave 173 replisome blokkerende lesjon PLA nærhetsligeringsanalyse DNA interstrand tverrbindinger antigenmerkede tverrbindinger
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