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Bioengineering
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使用高通量微流体设备生成动态环境条件
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
Please note that all translations are automatically generated.
Click here for the English version.
使用高通量微流体设备生成动态环境条件
DOI:
10.3791/61735-v
•
14:48 min
•
April 17, 2021
•
Bingchen Che*
1
,
Jie Zhu*
1
,
Dan Sun
,
Xiaoqiang Feng
,
Ce Zhang
1
State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology
,
Northwest University
Chapters
00:00
Introduction
02:23
Microfluidic Chip Design
04:33
Chip Fabrication and Operation
05:32
Microfluidic Chip Production using Soft Lithograph
07:03
Chip Operation
07:59
Generation of Dynamic Inputs in Cellular Microenviroments
08:43
Setup for High Throughput Live-cell Imaging
10:44
Automatic Data Analysis
11:42
Discussion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们介绍了一个用于复杂生命机械高吞吐量研究的微流体系统,该系统由 1500 个培养单元、一系列增强的永久泵和现场混合模组组成。微流体芯片可分析体内高度复杂和动态的微环境条件。
Tags
Microfluidic Device
High-throughput
Dynamic Environmental Conditions
Cell Culture
Peristaltic Pump
Shear-free Culture
Stem Cell Differentiation
Organ-on-chip
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