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Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples…
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Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
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Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
October 2nd, 2020
•
Vasudeva Bhat1,2, Cory Lefebvre1,2, David Goodale1, Mauricio Rodriguez-Torres1,2, Alison L. Allan1,2,3,4
Chapters
Summary
This experimental protocol describes the isolation of BCSCs from breast cancer cell and tissue samples as well as the in vitro and in vivo assays that can be used to assess BCSC phenotype and function.
Tags
Isolation Functional Assessment Breast Cancer Stem Cells Cell And Tissue Samples In Vitro In Vivo Characterization Assays Molecular Events Tumorigenicity Metastasis Therapeutic Strategies Single Cell Suspension Cell Dissociation Solution Culture Medium ALDH Substrate Buffer Tissue Sample Surgical Blades Dissociation BufferRelated Videos
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