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JoVE Journal Neuroscience
Génération de neurones humains et d’oligodendrocytes à partir de cellules souches pluripotentes pour la modélisation des interactions neurone-oligodendrocytes
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Génération de neurones humains et d’oligodendrocytes à partir de cellules souches pluripotentes pour la modélisation des interactions neurone-oligodendrocytes

Article DOI: 10.3791/61778-v • 10:53 min • November 9th, 2020
November 9th, 2020 •
*1, *1,2, *3, 1, 1, 3, 3,4, 1,5,6
1Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 2Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 3Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 4Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 5Department of Neurology, Warren Alpert Medical School of Brown University, 6Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University
* These authors contributed equally

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Les interactions neurone-gliale dans la neurodégénérescence ne sont pas bien comprises en raison d’outils et de méthodes inadéquats. Ici, nous décrivons des protocoles optimisés pour obtenir des neurones induits, des cellules précurseurs d’oligodendrocytes et des oligodendrocytes à partir de cellules souches pluripotentes humaines et fournissons des exemples des valeurs de ces méthodes dans la compréhension des contributions spécifiques au type cellulaire dans la maladie d’Alzheimer.

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Neurosciences numéro 165 cellules souches pluripotentes induites cellules iPS cellules souches embryonnaires humaines cellules ES neurones oligodendrocytes cellules progénitrices d’oligodendrocytes OPC maladie d’Alzheimer peptides bêta-amyloïdes synapses
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