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Generación de neuronas humanas y oligodendrocitos a partir de células madre pluripotentes para modelar las interacciones neuronal-oligodendrocitos
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1Department of Molecular Biology, Cell Biology and Biochemistry,Brown University, 2Department of Neurology,The Third Affiliated Hospital of Sun Yat-Sen University, 3Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine, 4Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 5Department of Neurology,Warren Alpert Medical School of Brown University, 6Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science,Brown University
Chapters
- 00:05Introduction
- 00:37Human Neuron Induction from Human Pluripotent Stem Cells
- 03:59Human Oligodendrocyte Precursor Cell (OPCs) Induction from Pluripotent Stem Cells and Oligodendrocyte Maturation
- 07:05Co-culturing of Human Induced Neurons (Ins) and Oligodendrocyte Precursor Cells (Iopcs)
- 08:06Results: Direct Generation of Human Induced Neurons from Human Pluripotent Stem Cells
- 10:23Conclusion
Las interacciones neurona-glial en la neurodegeneración no se comprenden bien debido a herramientas y métodos inadecuados. Aquí, describimos protocolos optimizados para obtener neuronas inducidas, células precursoras de oligodendrocitos y oligodendrocitos a partir de células madre pluripotentes humanas y proporcionamos ejemplos de los valores de estos métodos para comprender las contribuciones específicas del tipo de célula en la enfermedad de Alzheimer.
