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Label-Free Imaging af Lipid Storage Dynamics i Caenorhabditis elegans ved hjælp af stimuleret Raman Scattering Mikroskopi
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Label-Free Imaging af Lipid Storage Dynamics i Caenorhabditis elegans ved hjælp af stimuleret Raman Scattering Mikroskopi

Article DOI: 10.3791/61870-v • 10:59 min • May 28th, 2021
May 28th, 2021 •
1, 1, 1, 1,2,3
1Huffington Center on Aging, Baylor College of Medicine, 2Department of Molecular and Human Genetics, Baylor College of Medicine, 3Howard Hughes Medical Institute, Baylor College of Medicine

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Stimuleret Raman spredning (SRS) mikroskopi giver mulighed for selektiv, etiket-fri billeddannelse af specifikke kemiske moieties og det er blevet effektivt ansat til at billedet lipid molekyler in vivo. Her giver vi en kort introduktion til princippet om SRS mikroskopi og beskrive metoder til dets anvendelse i billedbehandling lipid opbevaring i Caenorhabditis elegans.

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Biologi Raman imaging Stimuleret Raman Scattering mikroskopi SRS mikroskopi C. elegans lipid metabolisme lipider fedt lipid dråber etiket-fri billeddannelse lipid imaging levende billeddannelse
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