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Hurtig antimikrobiel følsomhedstest ved stimuleret Raman-spredningsbilleddannelse af deuteriuminkorporering i en enkelt bakterie
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Hurtig antimikrobiel følsomhedstest ved stimuleret Raman-spredningsbilleddannelse af deuteriuminkorporering i en enkelt bakterie

Article DOI: 10.3791/62398-v • 12:08 min • February 14th, 2022
February 14th, 2022 •
1,2, 3, 1,2,4,5
1Department of Electrical and Computer Engineering, Boston University, 2Boston University Photonics Center, Boston University, 3Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 4Department of Biomedical Engineering, Boston University, 5Department of Chemistry, Boston University

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Denne protokol præsenterer hurtig antimikrobiel følsomhedstest (AST) assay inden for 2,5 timer ved enkeltcellestimuleret Raman-spredningsbilleddannelse af D2O-metabolisme. Denne metode gælder for bakterier i urin- eller fuldblodsmiljøet, hvilket er transformerende for hurtig enkeltcellet fænotypisk AST i klinikken.

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