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Brug af enkeltormdata til at kvantificere heterogenitet i Caenorhabditis elegans-bakterielle interaktioner
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Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions

Brug af enkeltormdata til at kvantificere heterogenitet i Caenorhabditis elegans-bakterielle interaktioner

DOI:
10.3791/64027-v

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09:54 min

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July 22, 2022

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, ,
1Department of Biology,Emory University, 2Department of Physics,Emory University

Chapters

  • 00:05Introduction
  • 00:47Preparation and Chemical Permeabilization of Worms
  • 03:41Mechanical Disruption of Individual Worms
  • 07:28Results: Heterogeneous Bacterial Colonization of the C. elegans Intestine
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en 96-brønds forstyrrelse af individuelle bakterielt koloniserede Caenorhabditis elegans efter kold lammelse og overfladeblegning for at fjerne eksterne bakterier. Den resulterende suspension er belagt på agarplader for at muliggøre nøjagtig kvantificering af bakteriebelastningen i et stort antal individuelle orme.

Tags

Keywords: Caenorhabditis ElegansBacterial InteractionsSingle-worm DataMechanical Disruption96-well FormatMedium ThroughputQuantificationWorm MeasurementsM9TX01OP50SDS/DTT SolutionChemical PermeabilizationWorm Cuticle

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