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JoVE Journal Bioengineering
Kvantificering af Fibrillar Kollagen organisation med Curvelet Transform-baserede værktøjer
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Kvantificering af Fibrillar Kollagen organisation med Curvelet Transform-baserede værktøjer

Article DOI: 10.3791/61931-v • 07:58 min • November 11th, 2020
November 11th, 2020 •
1, 1,2,3
1Laboratory for Optical and Computational Instrumentation, Center for Quantitative Cell Imaging, University of Wisconsin-Madison, 2Departments of Medical Physics and Biomedical Engineering, University of Wisconsin-Madison, 3Morgridge Institute for Research, Madison, Wisconsin, 4test, test

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Her præsenterer vi en protokol til at bruge en curvelet transform-baseret, open source MATLAB software værktøj til kvantificering fibrillar kollagen organisation i den ekstracellulære matrix af både normale og syge væv. Dette værktøj kan anvendes på billeder med kollagenfibre eller andre typer linjelignende strukturer.

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Bioengineering tumor mikromiljø ekstracellulær matrix kræft kollagen fiber organisation fibrillar kollagen kvantificering curvelet transformering anden harmoniske generation mikroskopi billedanalyse software
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