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JoVE Journal Bioengineering
Kvantifisere fibrillar kollagenorganisasjon med kurve transformerebaserte verktøy
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Kvantifisere fibrillar kollagenorganisasjon med kurve transformerebaserte verktøy

Article DOI: 10.3791/61931-v • 07:58 min • November 11th, 2020
November 11th, 2020 •
1, 1,2,3
1Laboratory for Optical and Computational Instrumentation, Center for Quantitative Cell Imaging, University of Wisconsin-Madison, 2Departments of Medical Physics and Biomedical Engineering, University of Wisconsin-Madison, 3Morgridge Institute for Research, Madison, Wisconsin, 4test, test

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Her presenterer vi en protokoll for å bruke et kurveglassede, åpen kildekode MATLAB-programvareverktøy for å kvantifisere fibrillar kollagenorganisasjon i den ekstracellulære matrisen av både normalt og sykt vev. Dette verktøyet kan brukes på bilder med kollagenfibre eller andre typer linjelignende strukturer.

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Bioengineering tumormikromiljø ekstracellulær matrise kreft kollagenfiberorganisasjon fibrillar kollagen kvantifisering kurve forvandle andre harmoniske generasjon mikroskopi bildeanalyse programvare
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