A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Cardiovascular and Metabolic Sciences, Lerner Research Institute; Women’s Health Institute,Cleveland Clinic, 2Women’s Health Institute,Cleveland Clinic, 3Department of Obstetrics and Gynecology, 983255 Nebraska Medical Center,University of Nebraska Medical Center, 4Anatomic Pathology,Cleveland Clinic, 5Gynecologic Oncology, Women’s Health Institute and Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine,Cleveland Clinic
Chapters
- 00:04Introduction
- 00:53Donor and Recipient Mouse Preparation and Donor Mouse Endometrial Tissue Harvest
- 02:59Peritoneal Tissue Fragment Injection
- 04:03Endometriotic Lesion Harvest
- 04:51Endometriotic Lesion Scoring
- 05:58Results: Representative Endometriosis Lesion Histopathological Analyses
- 06:39Conclusion
Muitos modelos de roedores de endometriose são limitados pela complexidade técnica, reprodutibilidade e/ou necessidade de animais imunocomprometidos ou ratos repórteres especiais. Apresentamos um sistema simplificado de indução de lesão utilizando qualquer camundongo experimental com um sistema de pontuação objetiva e independentemente verificável e sem necessidade de cirurgia de ovariectomia ou sobrevivência.
